题目：TARGETED SEQUENCING OF HUMAN GENOMES, TRANSCRIPTOMES AND METHYLOMES
报告人：  Jin Billy Li
Research Fellow
Department of Genetics, Harvard Medical School
主持人：席建忠
时间：12月22日（周一）上午10：00
地点：2138cn太阳集团古天乐廖凯原楼2-102（会议室）

报告内容摘要：
Next generation sequencing technologies have brought the cost down by at least two orders of magnitude. However, it is still prohibitively costly to sequence the full human genomes, transcriptomes, and methylomes. Specifically capturing and amplifying genomic regions of interest in parallel enables us to fully utilize the power of new sequencing technologies. We developed a padlock-based approach to amplify 55,000 exons in a single reaction and have continuously improved the capturing efficiency by ~10,000-fold. In addition to specifically capture genomic features such as exons, simultaneous targeting of the same sites in both genomic DNA and cDNA allowed us to identify allele-specific gene expression patterns and hundreds of novel A-to-I RNA editing sites. Because of the near 100% specificity of the padlock approach, we extended our technology to bisulfite converted human genomic DNA. Despite the lowered genomic complexity, we were also able to accurately measure cytosine methylation level at 10,000 CpG loci in a single capturing and sequencing reaction. Interesting biological discoveries will also be discussed.

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联系人：席建忠，010- 62760698