题目：Understanding and Re-engineering Nucleoprotein Machines to Cure Human Disease
报告人：Dr. William S. Dynan
Professor and GRA Eminent Scholar, Medical Collegeof Georgia,
Associate Director, Nanomedicine Center forNucleoprotein Machines

主持人：方竞　教授 
时间：2月１７日（周二）下午４:30
地点：力学楼314会议室

报告内容摘要：
The mammalian nucleus is filled with self-organizing, nanometer-scale　nucleoprotein machines that carry out DNA replication, RNA biogenesis　and DNA repair. We take, as a model, the nonhomologous end-joining　(NHEJ) machine, which repairs radiation-induce DNA double-strand　breaks. The NHEJ machine consists of six core polypeptides and 10-20　ancillary polypeptides.  We are developing a NanoToolbox that includes　bright, stable, biocompatible fluorophores, efficient protein and　nucleic acid-tagging strategies, and sensitive, high-resolution　imaging methods that will allow us to visualize the behavior of single　NHEJ complexes in living cells.  We have also designed and synthesized　an antibody fragment with properties that allow it to enter cells by　nondestructive receptor-mediated endocytosis, escape the endocytic　compartment to enter the nucleus, bind a regulatory site in the NHEJ　machine, and sensitize human cancer cells to a clinically relevant　dose of ionizing radiation. Results suggest a potentially general　approach for influencing the activity of the NHEJ machine and adapting　it to new purposes, including gene correction in stem cells.

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