讲座题目：Probing Nucleosome Function: Systematic Mutagenesis Study of Synthetic Histones in Saccharomyces Cerevisiae

报告人：Junbiao Dai

时 间：9月17日下午1:00-2:30
地 点：北大校医院A-534
主持人：席建忠（博士）

报告内容摘要：
      The structural integrity of the nucleosome is central to regulation of DNA metabolism and transcription. Using a synthetic approach, a library of 486 systematic histone H3 and H4 substitution and deletion mutants in Saccharomyces cerevisiae was generated that probes the contribution of each residue to nucleosome function and can be episomal or genomically integrated. We probed fitness contributions of each residue to chemical perturbagens of chromosome integrity and transcription, mapping global patterns of chemical sensitivities and requirements for three forms of transcriptional silencing onto the nucleosome surface. Each histone mutant was tagged with unique molecular barcodes, facilitating identification of histone mutant pools through barcode amplification, labeling, and TAG microarray hybridization. We used the barcodes to score complex phenotypes including competitive fitness in a chemostat, DNA repair proficiency, and synthetic genetic interactions, revealing new functions for distinct histone residues and new interdependencies among components of nucleosomes and their modifiers.

报告人简介：

EUCATION