讲座题目：Characterizing mechanical response of neo-tissue andcells 

报告人：Dr. Jinju Chen

时 间：12月17日（星期五）下午3:00-4:00
地 点：校医院A-534会议室
主持人：熊春阳

报告内容摘要：
     1) Nanomechanical characterization of neo-bone formation on Ti alloy
     The long term clinical success of an orthopaedic implant is strongly related to bone formation at the biomaterial-tissue interface. Surface parameters that include topography or surface roughness influence this process of osseointegration. Electropolishing is a cost effective approach to modify the surface of metals such as titanium. Indeed improved osteoblast spreading and enhanced bone formation in terms of quantity has been observed on electropolished Ti alloy surfaces. However, the maturity of the neo-bone is of critical importance and can be evaluated using nanoindentation techniques. In this study, the mechanical properties of the neo-bone formed on Ti alloy will be assessed by nanoindentation in association with in-situ scanning probe microscope (SPM). These studies have confirmed that the enhanced bone formation on electropolished surface provide an increased mechanical maturity.
      2) Cell traction field
      The complex three dimensional form of the heart is shaped by multiple cell types that include endothelial cells, cardiomyocytes, neural crest cells, smooth muscle cells and fibroblasts. The force generated during cell-cell and cell- matrix interaction is important in heart formation and its function. At a cellular level, the transduction of these forces provides feedback to regulate growth and development, via the core regulatory gene networks. In this study, cell traction force microscopy (CTFM) and finite element analysis (FEA) was utilised to determine the exerted traction forces of individual fibroblasts and cardiomyocytes on a hydrogel substrate.
      3) A viscoelastic model to predict chondrocyte deformation seeded in agarose during cyclic compression
      Chondrocytes respond to loading by altering synthesis & catabolism of matrix. These responses are both frequency and duration- dependent. Cell deformation may be involved in mechanotransduction, but its nature cannot be fully revealed by an experimental approach alone. In this study, a computational model has been developed to investigate both the chondrocyte deformation within 3D hydrogel scaffold and the associated stress evolution. In addition, the important of interface adhesion between the cell and scaffold has been addressed.