讲座题目：Stiffness variation in human cells and high-throughput methods to separate by stiffness

报告人：Dr.  Todd Sulchek

时 间：9月15日（周四）下午2:00-3:00
地 点：北大校医院A-534会议室
主持人：熊春阳（教授）

报告内容摘要：
     The biophysical properties of human cells may vary with different cell types or may vary within a single cell type due to disease. We will discuss the variation of cell stiffness due to expression changes within ovarian epithelial cells and how this relates to ovarian disease. For these experiments we utilize atomic force microscopy (AFM) stiffness measurements. We will also discuss a new microfluidic technique for continuous cell separation utilizing variation in individual cell stiffness. A microfluidic channels decorated by periodic diagonal ridges which force cells of different stiffness values to follow different trajectories. We demonstrate stiffness dependent separation by introducing a range of concentrations of a cell softening agent called cytochalasin D to lymphoblastic cells. The agent produces cells which vary in mechanical stiffness. By flowing these cells through the microfluidic device, we can quickly and efficiently separate mixtures into subpopulations of stiff cells and soft cells. We will then summarize how we expect this technology may produce new biomedical diagnostic capabilities.