题目：Engineering Clostridial Fermentation for Biofuels and Chemicals Production
报告人：Shang-Tian Yang
时 间：1月8日（周二）下午2:00
地 点：澳门太阳娱乐网站官网1号楼210会议室
报告内容摘要：
This presentation will focus on the recent progress in metabolic engineering of Clostridia for economical production of biofuels, including butanol from renewable biomass. Butanol is an important industrial solvent and potentially a better transportation fuel than ethanol. Currently, butanol is almost exclusively produced via petrochemical routes. Biobutanol will have a great potential to compete with ethanol as a transportation fuel when its production cost is reduced by using advanced fermentation technologies such as metabolically engineered Clostridia mutants. Conventional acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum or C. beijerinkii is severely limited by its poor butanol yield (<0.25 g/g glucose), low productivity (<0.5 g/L?h), low final butanol concentration (<15 g/L), and poor process stability. Recently, we have developed several metabolically engineered mutant strains of clostridia for butanol production. A novel solventogenic Clostridium was developed by introducing butanol biosynthesis pathway and blocking acid producing pathways in non-solventogenic acid-forming Clostridium tyrobutyricum which has relatively simple metabolic pathways with high flux toward butyryl-CoA, the precursor for butanol biosynthesis, and high butanol tolerance. A high butanol titer of >16 g/L with a high butanol yield of 0.33 g/g substrate were obtained in batch fermentation. In addition, we have also developed a mutant strain of Clostridium acetobutylicum with high butanol tolerance and productivity through evolutionary engineering in a fibrous bed bioreactor (FBB). The high butanol tolerant strain JB200 can produce up to 21 g/L butanol in conventional free-cell fermentation and 28.2 g/L butanol in the FBB fermentation. When the mutant strain is used in fermentation coupled with continuous gas stripping for in situ butanol recovery, high butanol productivity and yield can be achieved with an extremely high butanol titer of >150 g/L in the gas stripping condensate that can be easily purified with a low energy input.
报告人简介：
Shang-Tian Yang is Professor of Chemical and Biomolecular Engineering at the Ohio State University and the Director of Ohio Bioprocessing Research Consortium, which works with industry in developing novel bioprocesses for economical production of value-added products from food processing wastes and agricultural commodities. Dr. Yang has over 180 scientific publications and a dozen of US patents in the bioengineering field. He is the editor of the book “Bioprocessing for Value-Added Products from Renewable Resources: New Technologies and Applications” published by Elsevier in 2007. He is also a co-founder of two biotechnology startup companies. Dr. Yang is an elected fellow of American Institute of Medical and Biological Engineering and an active member of American Institute of Chemical Engineers (AIChE) and American Chemical Society (ACS). He also serves as an Associate Editor for the journal Process Biochemistry. Dr. Yang received his Ph.D. degree in Biochemical Engineering from Purdue University in 1985 and B.S. degree in Agricultural Chemistry from National Taiwan University in 1976.